![]() Their effector module is a single multidomain protein (Cas9 in type II). They have different effector modules, composed of multiple Cas proteins. (*) based on the identification of signature genes for CRISPR–Cas types and subtypes.ĬRISPR-Cas systems are thus classified into 2 classes, 6 types and 33 subtypes. We present here the classification of Kira Makarova & al. This precursor is then matured into small crRNA that play a role in the targeting and destruction of homologous foreign sequences.Īll cas genes (13 plus many variants) are not shared by all CRISPR–Cas systems, leading to an enormous diversity. This is further shown by their presence on megaplasmids.ĬRISPR loci are transcribed into a pre-crRNA from the leader acting as a promoter. Phylogenetic studies performed on the CAS protein suggest that CRISPRs are acquired by horizontal transfer. Their number varies from one type to another. Perform the three different functions of the immune system: adaptation, crRNA maturation and interference. Several genes called cas for CRISPR-associated are found in the vicinity of CRISPRs and viruses, plasmids or mobile genetic elements. The unique sequences or spacers correspond mostly to fragments of foreign DNA, ie. The spacers in the different CRISPRs are different. In a given strain several CRISPRs can be found with a single or different repeat sequences but only one of each kind is associated Together with a set of genes called cas for “CRISPR-associated”, they constitute an immune system.ĬRISPR-associated genes are genes found closely linked to the repetitive sequences. The CRISPR locus is generally flanked on one side by an AT-rich leader sequence of 100-350 bp,Īcting as a promoter for the pre-crRNA synthesis. Sometimes, at one end of the CRISPR, the repeat is not totally conserved, it is called degenerate repeat. They have been described in a wide range of prokaryotes, including the majority of Archae and many Eubacteria.Ī CRISPR is a succession of 23-47bp sequences called repeats separated by unique sequences of a similar length (spacers). That show characteristics of both tandem and interspaced repeats. ( CRISPRs) are specific structures found in many prokaryotic genomes Clustered Regularly Interspaced Short Palindromic Repeats
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![]() Furthermore, the CMYK values for pastel purple are (30,34,0,0) almost parallel to the actual percentages. Whereas the RGB values focus on a 3-color combination, the CMYK values focus on 4-color combinations. ![]() The CMYK Values and Percentages for Pastel Purple In the RGB (red, green, blue) system, the pastel purple color percentage is comprised of pastel purple in the RGB system is (177,162,202). The RGB Values and Percentages for Pastel PurpleĮach system has a different value, or percentage of colors, that make up every color in the graphic design spectrum, and the same can be said for pastel purple. Thankfully, the HEX value for pastel purple is simple the code you need to input is #B1A2CA. The HEX color system is popular in many graphic design centers, so if you work in the industry there’s a good chance you’re completing your projects based on this spectrum. Keep reading this article to find out more! The Pastel Purple Color Code: The HEX Code You can easily create the pastel purple color using the pastel purple color code specific to the type of program you’re running, and this article talks about the specific code that you need as well as the colors that make up this brilliant color. Luckily for you, you don’t have to go it alone. This beautiful color is a popular choice for many clients and artists alike, but even with this being the case it is a complex color to create in any graphic system, and you could end up creating one of the many other types of purple if you don’t know what you’re doing. Though most of the time it can be easy to select the color that you desire, it’s not uncommon that you run into a situation where you need more complex and specific swatches for your task.Ī perfect example of this is the color of pastel purple. Whether you’re a professional graphic designer or an amateur artist, finding the right color is essential for pulling off your graphic artwork. If you would like to use Digital Dollface design assets for a small business, other than supplying educational tools and classroom material, please contact me for a different licensing agreement at: kelleybrock(at)gmail(dot)comĬopyrighted © 2014 Digital Dollface - All rights reserved.#AEC997 Pastel Purple Color Swatch Sample Please be sure all files are flattened & artwork is merged properly with documents. (Here are instructions on how to place a link on Teachers Pay Teachers)Ģ) You may NOT use artwork digitally in any way that can be easily copied or downloaded and / or distributed. You may use my products to create lesson plans, teacher's materials & learning tools for online resale, with the following requirements:ġ) You must give credit to Digital Dollface, and provide a direct link to my shop, in the following way: Artwork courtesy of Digital Dollface You may use my products for personal use, or in classrooms for educational purposes as you wish. Jpg format, 1 zip file This is one of the 12 colors in my PASTEL collection! The collection of 12 harmonious colors allow you to mix and match easily, and coordinate your digital background patterns with same-color clip art elements! Terms of Use: Please note, this is a digital, downloadable product and no physical product is for sale. Pastel Purple Background Patterns / Lavender Digital Paper ![]() In many ways, music is a universal language, crossing borders and opening up a whole new world to our minds. You can follow the link and listen to the rhythm before beginning to play. This pedal on the piano adds a legato effect to your music, helps connect notes and chords together, and smoothen out transitions. Prodaja svih vrsta muzickih instrumenata i pratece opreme u Beogradu, Novom Sadu, Nisu, Subotici. If your thumb piano has flat keys, it is recommended that you use classic sheet music for the piano.Īlso, we have added a QR code to all songs. Online prodavnica muzickih instrumenata Mitros Music. Some melodies might have been changed and simplified to be played within one octave. Our sheet music is universal and suitable for any 8-, 10- or 17- note kalimba or mbira. It is easier to play the finger piano by following numbers. If you are a beginner, playing by notes can be difficult. This educational book will help you begin to play music easily. You will enjoy folk music, children's songs, hymns, popular melodies, and dances. ![]() Tip: The Kalimba has three alternate layouts. Try swiping quickly over multiple tines in a horizontal fashion to play chords. Tip: To play the Kalimba, hover your pointer over the tines, to 'pluck' them much like a real Kalimba is played. In the case of the keyboard, you can see the valid keys by displaying the keys in Option>Display settings. ![]() You can play notes by clicking with the mouse. Its not a game, but I made it with Construct3 game engine. The songbook is colorfully designed with national patterns. The Kalimba spans a full two octaves from B3 to B5. It plays a simple but very rich sound tone. Their goal was to assist you in acquainting you, your kids, or students with the musical traditions of diverse nations and cultures. This book includes complete melodies as well as substantial segments of uncomplicated, famous traditional folk songs from 30 different countries. We've gathered 45 different national songs adapted especially for kalimba, including lyrics, classical sheet music, number-coded notation, and online audio tracks. Kalimba Sheet Music Online tuner For guitar, ukulele. The Dragonborn Comes (from The Elder Scrolls: Skyrim). International songs are one of the most exciting and appealing avenues to introducing children to new and different cultures through music. Best Kalimba Songs of All Time (Free Kalimba Tabs Kalimba Time Making Music Fun. Download sheet music and search pieces in our sheet music database. Learn easy beginner kalimba songs with simple kalimba arrangements. ![]() We constantly focus on better understanding our customer, that's the ultimate goal. What will we see next from REVOLVE, and more specifically, from you?Ĭontinuing to nurture and explore our influencer marketing strategy. The constant change and evolution of fashion and technology keeps us excited, there's on-going opportunity if you welcome change with an open mindset. What continues to excite you about working in the fashion/tech/e-comm industry? Stay knowledgeable and informed in market changes but don’t put your energy into copying others. What mistakes have you learnt from and benefited from in your career?ĭon't buy into the hype and stay focused on what makes sense for you and your brand. If you can surround yourself with good people, you'll always succeed. It's important to support one another, encourage taking risks but also ensure your potential losses are as minimal as possible. One of our largest experiential marketing efforts is #REVOLVEawards, an annual celebration of the influencers, talent, and brands in our network that are paving the way in our industry. What can we do to encourage women to take the leap and launch their big ideas?Ĭontinue to offer a platform to celebrate female empowerment through brands we carry, and the influencers we work with. We constantly thrive on change and aim to stay ahead of the curve when it comes to influencer strategy and communicating our brand message. We see ourselves always working with press, influencers, and other forms of digital media. REVOLVE was one of the first fashion brands to work closely with influencers-how do you see your relationship with traditional media and influencers evolving from here? At the same time, we're conscious to follow our intuition and focus on what feels right for our brand. Everything we do is data driven: from the brands we carry to the influencers we work with, to the content we post on our social channels. We've always tested strategies in small ways and scaled from there. Social media is always evolving-look how far we’ve come in the last decade! How does REVOLVE continue to pioneer in this space? Do you trust your gut instincts? Do you make decisions based on data? A mixture of both?Ī mixture of both. I was so young, it felt like there was nothing to lose. In 2008, we expanded the business to include a luxury fashion offering with our sister site FORWARD by Elyse Walker and later on acquired Alliance Apparel, our in-house design branch.Īfter the early-00’s dot-com bust, how did you shake any fear to launch an e-commerce platform? Talk us through that light-bulb moment. In 2003, we started REVOLVE with $50,000 of our savings, working out of our apartment and using it to house all the stock for the site. I started at a software start-up as a business analyst in 2000 where I met my business partner. Talk us through what you were doing before you launched Revolve? What were some of the roles you held? While recently in Australia to launch REVOLVE into this market (free shipping on orders over $100 USD and free returns), we sat down with co-founder Michael Mente to learn more about the brand's success, how he thinks about the future of fashion, and what's next. The influencer still works with the brand to this day. One of the first influencer collaborations REVOLVE worked on was with Rumi Neely of Fashion Toast. REVOLVE has been working closely with influencers for just over a decade, with a strategy firmly rooted in data and analytics. The hashtag is part of REVOLVE's unique approach to influencer marketing, where essentially the brand takes a roster of influencers on a holiday to a beautiful location, with the goal to have your Instagram feed taken over by lust-worthy holiday snaps (and outfits, of course). REVOLVE is an e-commerce powerhouse, stocking over 700 brands across fashion and beauty, and host to one of the most drool-worthy hashtags on Instagram- #REVOLVEaroundtheworld (68,000 posts and counting). In case you're not familiar with REVOLVE, let us introduce you. ![]() ![]() The visual scope itself appears much wider than the first game as it moves away from the self-contained vessel of the Maw. While Little Nightmares II is very much more of the same with bigger bells and whistles, that doesn’t necessarily mean that it substitutes any blind lack of extra care and attention. This is a sequel to a successful entry point after all, so expect bigger production and a higher emphasis than what arrived previously. While those new to the series could easily dive in and work their way backwards, the subtleties of certain revelations will surely make more of an impact if played in order. Those who are a fan of the first game will undoubtedly soak up all interpretation of lore this sequel has to offer. ![]() Whether it be the result of the eerie effect of stop-motion style frames clapping together or the sense of one of those dreams where the faster you try to run, the heavier your legs become. All I am willing to share is the feeling I had. In any case, I flat out refuse to mention anything at all that could spoil what lies ahead. After all, in looking back, the marketing team seemed more than willing to spill some beans in the name of promotion. I could, and probably should, pen down some examples of standout moments in this Little Nightmares II review to provide a tease of what to expect in this doll-house horror of a sequel. By simply merging the atmosphere of excellent sound, intelligent camera work, clever animation and possessing the body of a pre-adolescent, this genius recipe manages to steam over most efforts that the movie industry has attempted in the last 20 years or so. Our childhood, and the potent imagination that came with it. Little Nightmares II has a cunning tendency to pray on a common fear that we all share. ![]() i would need you guys to be involved this time. Please do leave a comment so we would know what we need to be fixed. (the restart when you lose is quite buggy. once your slot is filled then you can shoot back. it will be filled evertime you absorb any projectile. ![]() Right beside the shield, you can see 6 squares, that would be your inventory. the next time you get hit, it will be deducted to your HP. You can see the shield's status below is the circle with the blue color. if you lose all shield, you're pawned !! but try not get hit a lot cuz the shield is being generated overtime. you will need to use the ship's shield to absorb projectiles and shoot it back to the enemy. the ship in this stage has no fire arms yet. i wanted to post this just to let you guys try it out before i proceed deeper to the develpoment of the game i must note, that this is not the complete game. this is just a test to know what you think of the game. If you like my work and like to support the project, click HERE!!!įor those who's new to this blog and this project, you can see this link HERE!!! So what do you think? please let me know by leaving a comment so far, this project is attracting hentai fans and non-hentai fans which is good. basically, there would be 2 games but works as one project. then fully cell shaded on the hentai parts. Since it would be separated, i may leave the main games's cinematics as a flat colored since that part is really time consuming too. which can also reduce my workload and the programmer too like a filler game which also part of the story, but optional to be played. one idea is to have the hentai stuff outside of the main game itself. im potentially putting my self at risk both legally and production related. Īnyway, i'm getting some advise from different people regarding my project since it has some NFSW i hope you guys would stick around till i get back. Got some real life issues atm and it really needs attention really bad. Īfter this post i will find my self chained to the comissions once again. people like you are the reason why this blog still exist. this is the hardest part to be honest, "promotion". i need to be active on other sites and raise awareness. This is the first cinematics for the game. If you like my work and generous to help us out, click HERE To all the new comers on this project, you can click HERE altough it will take awhile so please be patient. owner was easy to talk to, and agreed enthusiastic. what makes this exciting is it's gonna be a crossover with Eyline Avari. i am also working on a the leica for these Dracos. i'm almost done revising my script for ACT 2 and 3. i said in my old post, i not making hardcore furry. if you noticed, i kept the "human like" looks on these characters. and there's a lot of hentai stuff with them LOL. if there's a protagonist, then these guys are the antagonist. yes these characters are gonna play an important role in the RC. just a another type of reptile specie, and they can fly :D. these are reptilian race which kouia refers to as, " lizards ". ![]() or you can email me, so i got myself designing the dracos. please leave a comment down there if you want to see it :PĪnyway, if you're new to this blog and you like my work and like to support this project, you can visit my patreon campain HERE . but that's just me LOL !! i would need to hear you thought on this. posting it feels like it would lose interest for stage 2. i want to post a compiled version next time with scenes from ACT1,stage1,ACT2,stage2. i'm not decided yet wether if i would post the next cinematic tho. the only thing missing is the audio from a certain scene, i'm still waiting for the VA's recording atm. I am currently done with ACT 2 cinematics. Total file size is 5+mb so give it some time to load removed the bug/glitch when restarting stage 1 adjusted the shield cooldown from 10 to 4 seconds stage has been widen-up from 600圆00 to 800圆00 bottom line is, we made some changes to the previous version so we testing it again. sadly, he's been busy and i feel like i really need to post it anyway. i did'nt post this version earlier this month cuz i was waiting for my programmer to fix it. the hyperlink at the under-construction scene is not working properly. there are still a few things to fix here is the BG at the tutorial stage is going the other way, and the graphics in the inventory during actual gameplay. Okay guys, here is a new version of the Rogue Courier i am working at. This is a tiki drink I would have loved when I first started drinking tiki drinks, but a decade and a half in, this comes off bland to me. And if it’s going to be heavy on the juice, let it be exotic juices like pomegranate, passion fruit, pineapple, papaya, etc., not just orange and lemon. The orgeat adds a nice nuttiness to the drink, but the white rum, orange juice, and lemon juice are the most prominent flavors. ![]() I envision juice, booze, and spice when I think tiki, but this cocktail lacks spice. Again that is a personal opinion, and taste is subjective. The scorpion was Trader Vic’s third most popular cocktail, and while I think this is the best version of the drink, it’s not a top-tier tiki cocktail in my book. The ingredients are the same, but the volumes are different. Enjoy 50 percent off well drinks, house wines and domestic beers and discounted. A daily happy hour is offered from 3 p.m. A swanky old-school hang out, Peppermill has been delighting diners in Vegas since the 1970s. Oddly enough, the scorpion bowl, which is made to serve 3, is not just 3x the ingredients of the single-serve one. See detailed description, map, photos and videos of Peppermill restaurant in Las Vegas. But I will say those flaming scorpion bowls are a ton of fun. The recipe here is the single-serve version and, in my opinion, the best version of the drink. For comparison, a Jalapeno peppers come in at o. Trader Vic heavily modified the recipe over the years and, in his 1972 edition, added the scorpion bowl and a single scorpion cocktail. According to the tag, when I bought the plant, Trinidad Scorpion Peppers are 2 million + Scoville Heat Units. That original scorpion punch is also in the 1972 edition, but the updated edition included his more popular versions of the scorpion. Those are odd proportions like Trader Vic added the gin and mint as a joke. The original scorpion recipe was 1.5 bottles of rum, 2 oz gin, 2 oz brandy, 1 pint of lemon juice, 1/2 a pint of orange juice, 1/2 a pint worth of orgeat, 1/2 a bottle of white wine, and two sprigs of mint. That is why all of our trips are flightless in destination, fully carbon offset - and we have ambitious plans to be net zero in the very near future.The original scorpion was not a bowl or an individual cocktail but a punch from Victor Bergeron’s First book from 1947 and was a punch made for 12. We are committed to go as far as possible in curating our trips with care for the planet. We know that many of you worry about the environmental impact of travel and are looking for ways of expanding horizons in ways that do minimal harm - and may even bring benefits. Our Private Trips are fully tailored itineraries, curated by our Travel Experts specifically for you, your friends or your family. Our Rail Trips are our most planet-friendly itineraries that invite you to take the scenic route, relax whilst getting under the skin of a destination. Our Trips are suitable for both solo travelers, couples and friends who want to explore the world together.Ĭulture Trips are deeply immersive 5 to 16 days itineraries, that combine authentic local experiences, exciting activities and 4-5* accommodation to look forward to at the end of each day. That is why we have intensively curated a collection of premium small-group trips as an invitation to meet and connect with new, like-minded people for once-in-a-lifetime experiences in three categories: Culture Trips, Rail Trips and Private Trips. Increasingly we believe the world needs more meaningful, real-life connections between curious travellers keen to explore the world in a more responsible way. We are proud that, for more than a decade, millions like you have trusted our award-winning recommendations by people who deeply understand what makes certain places and communities so special. ![]() Since you are here, we would like to share our vision for the future of travel - and the direction Culture Trip is moving in.Ĭulture Trip launched in 2011 with a simple yet passionate mission: to inspire people to go beyond their boundaries and experience what makes a place, its people and its culture special and meaningful - and this is still in our DNA today. ![]() Coat the slices with a thin layer of olive oil and place them in the hot chargrill pan or on a hot barbecue. Slice the fruit into rounds approximately 1cm in thickness (you should get 4 slices from each clementine). Next, prepare the charred clementines.Place in the water bath and leave to cook for 30 minutes. Seal the venison inside a vacuum bag using a chamber sealer.For the juniper seasoning, place the thyme, peppercorns and juniper berries in a pestle and mortar and reduce to a fine powder by grinding.Preheat the water bath to a temperature of 57⁰C.When the potato rösti mix is completely cold, take it out of the vacuum bag and divide into 4 triangular pieces of equal size.Purée, then strain through a fine sieve and chill in the fridge until needed. When the sugar has fully dissolved, pour the syrup into a blender and add the blanched peel. Combine the sugar, water and olive oil in a saucepan and cook over a gentle heat.Blanching the peel 3 times in this way will remove its bitterness. Repeat step 8 twice, using fresh water each time.After this time, drain off the cooking water, leaving the peel in the pan. Add just enough cold water to the saucepan cover the peel, then bring to a gentle simmer and cook for 20 minutes.Put the fruit to one side and place 300g of the peel in a saucepan. To make the clementine purée, remove the peel from the clementines.When the mixture reaches a sauce-like consistency, remove from the heat, strain through a fine sieve and leave to cool. Return the heat to medium, bring the mixture to the boil and reduce over a medium heat, skimming off any fat and impurities that rise to the surface. Turn up the heat and simmer until the wine has reduced by half, then mix in the chicken stock, thyme and peppercorns. ![]() ![]() Add the red wine and stir well to deglaze the pan.When the onions have caramelised, mix in the mushrooms and fry until soft and brown. In a large saucepan, warm the butter over a medium heat until melted, then stir in the onion and garlic and sauté. Cook in the water bath for 4 hours, then remove and allow to cool completely before opening the vacuum bag. Using a chamber sealer, seal the potato and duck fat mixture in a large vacuum bag.Heat the duck fat in a saucepan until melted, then stir into the potato.Wring the tea towel until you cannot squeeze out any more moisture, then tip the potato in a bowl. After 15 minutes, wrap the potato in a clean tea towel. The salt will draw the moisture out of the potato. In a colander, toss the grated potatoes with a generous pinch of salt, then place the colander in a sink or bowl and leave to drain.To make the potato röstis, preheat a water bath to a temperature of 80⁰C.Lay the meat slices out on a plate, top with the gremolata (if using), drizzle with extra-virgin olive oil, and squeeze the lemon wedges over the meat.Recipe courtesy of Will Holland from METHOD. ![]()
You have only 5GB of free storage in iCloud. You could upload photos to iCloud in 2 ways, via iPhone or computer. How to Download Photos from iCloud to iPhone via Īn Easy and Quick Way to Transfer Photos from iPhone to iPhone without iCloudīefore you download photos from iCloud to new iPhone 14, you should save photos to iCloud from old iPhone. How to Get Photos from iCloud to iPhone via iCloud Backup How to Get Photos from iCloud to iPhone via Photo Library There's another tool that can help you transfer photos from iPhone to another one with different Apple IDs. Fortunately, iCloud is not the only way to help you transfer photos. If you want to download photos to an iPhone with a different account, it will be inconvenient. ► To download photos from iCloud to iPhone, you need to log in to the Apple account where the photos are stored. ![]() Please make sure that you have a good Internet connection, otherwise, you may meet the iCloud photos not downloading issue. You can get photos from iCloud to iPhone in 3 ways. ICloud provides us an easy way to help us transfer data from old iPhone to new iPhone. It’s time to get your favorite new iPhone and enjoy the latest creative design but your beautiful photos should not be neglected when you start to use your new iPhone. Every year Apple would release a new series of products. IPhone has a lot of fans all over the world. ![]() I have about 1000+ photos on iPhone 8, so anyone could tell me how to get photos from iCloud to new iPhone? I want to transfer photos to my new iPhone 14 as soon as possible. How to Transfer Photos from iCloud to New iPhone Or how about his weird-ass issues with food? He wants the girl slim and in shape, yet he won't stop trying to force her to eat! I'm sorry, I just couldn't take any of this seriously. ^My inner goddess will cap yours in the face if you don't shut the fuck up^ Speaking of crap, if I ever, ever ever have to hear/read the words "inner goddess" again, I'm going to construct a pyre out of tampons and maxi pads, light it, and toss unsuspecting women into it. Crap, Holy Crap, Double and Triple Crap, Oh Crap, This Crap, That Crap, any and all Crap. oh my." "He began kissing a trail down my belly, oh my." "He's an insufferable douchenozzle, oh my!" (I'm just thankful that neither lions, tigers, nor bears were brought into this mess at any point.)Ībout halfway through, I wished I'd been keeping track of the word "crap" because Ana is constantly saying/thinking it. I went back and counted, and kept track throughout the rest of the book, and do you have any idea how many fucking times Ana said "Oh my" in this monumentally bad missive? Do you? I'll tell you 79! 79 motherfucking times. Turns out, I found something about 15% through. I can't think why.Īnyway, after reading about the description of Christian's building (hello, first penis metaphor), I had to sit through the awful dialogue between these smarmy idiots and hope beyond hope that something, anything, would distract me enough to see me through to the end. Any time an author tries to sell me on a character's "charm" by waxing hormonal about how "ridiculously good-looking" he is, I snicker inwardly. What were my first impressions of Christian? Well. My first impressions of Ana were bad (deservedly so). Once he gave up, however, I had to travel the rest of the road alone. The only thing that made the first 4% of this book tolerable was the fact that I read it aloud to my younger brother, and his frequent commentary was amusingly distracting. Unwelcome, apparently because her friend is beautiful, even when she has the flu.? What a petty, jealous cunt. My suspicions were confirmed a few pages later when Ana admits that any "sympathy" she feels for her sick friend is "unwelcome". It was cemented that early, my deep, deep dislike for this moronic, simpering asshole. "I scowl with frustration at myself in the mirror." I knew from the very first line this wasn't going to be good. Most of the time he's serious, brooding, and threatening. The only time Ana seems to like him as a person is when he's being "lovable", and those times are few and far between. ![]() Apparently, his only redeeming qualities are, in this order his ridiculous good looks, his money, and his giant penis. Fuckin' really? She "flushes" constantly, and on several occasions referred to her hoo-hoo-naughty place as "down there."Ĭhristian is a misogynistic, self-loathing, abusive piece of shit. At one point, she thinks putting her hair in pigtails will keep her safe from Christian's lusty advances. ![]() I'm fairly certain there hasn't been a woman this naive since 'round about 1954. She's a virgin (of course) who's never taken any sexual interest in anyone before. ![]() She's insecure to the point of it being laughable, 'klutzy' (even though she only trips twice in the entire book), and a complete ditz. I did read that.Īna is just a giant mess of a human being. What in the hell just happened? Did I really read that? Oh, my god, I did. |
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